| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| 10H2Lardelli_2_R1.fq.gz | 30,799,559 | 0 | 35-76 | 48 |
| 10H2Lardelli_R1.fq.gz | 31,038,353 | 0 | 35-76 | 48 |
| 11I1Lardelli_2_R1.fq.gz | 36,007,430 | 0 | 35-76 | 47 |
| 11I1Lardelli_R1.fq.gz | 36,272,664 | 0 | 57-76 | 47 |
| 12I2Lardelli_2_R1.fq.gz | 36,630,904 | 0 | 35-76 | 48 |
| 12I2Lardelli_R1.fq.gz | 36,932,023 | 0 | 62-76 | 48 |
| 1B1Lardelli_2_R1.fq.gz | 34,992,088 | 0 | 35-76 | 48 |
| 1B1Lardelli_R1.fq.gz | 35,234,870 | 0 | 60-76 | 48 |
| 2B2Lardelli_2_R1.fq.gz | 35,657,551 | 0 | 35-76 | 48 |
| 2B2Lardelli_R1.fq.gz | 35,918,051 | 0 | 49-76 | 48 |
| 3D1Lardelli_2_R1.fq.gz | 33,432,403 | 0 | 35-76 | 48 |
| 3D1Lardelli_R1.fq.gz | 33,708,122 | 0 | 46-76 | 48 |
| 4D2Lardelli_2_R1.fq.gz | 33,537,736 | 0 | 35-76 | 48 |
| 4D2Lardelli_R1.fq.gz | 33,760,555 | 0 | 44-76 | 48 |
| 5F1Lardelli_2_R1.fq.gz | 33,100,795 | 0 | 35-76 | 48 |
| 5F1Lardelli_R1.fq.gz | 33,349,938 | 0 | 35-76 | 48 |
| 6F2Lardelli_2_R1.fq.gz | 34,992,886 | 0 | 35-76 | 48 |
| 6F2Lardelli_R1.fq.gz | 35,240,827 | 0 | 35-76 | 48 |
| 7G1Lardelli_2_R1.fq.gz | 31,870,704 | 0 | 35-76 | 48 |
| 7G1Lardelli_R1.fq.gz | 32,106,359 | 0 | 60-76 | 48 |
| 8G2Lardelli_2_R1.fq.gz | 32,160,953 | 0 | 35-76 | 48 |
| 8G2Lardelli_R1.fq.gz | 32,413,448 | 0 | 51-76 | 48 |
| 9H1Lardelli_2_R1.fq.gz | 34,515,273 | 0 | 35-76 | 47 |
| 9H1Lardelli_R1.fq.gz | 34,758,354 | 0 | 58-76 | 47 |
Library Sizes ranged between 30,799,559 and 36,932,023 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Hsapiens Genome
GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Genome
Universal Adapter Content
Total overrepresented sequences for each library
## Overrepresented_sequences missing from 10H2Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 10H2Lardelli_R1.fq.gz## Overrepresented_sequences missing from 11I1Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 11I1Lardelli_R1.fq.gz## Overrepresented_sequences missing from 12I2Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 12I2Lardelli_R1.fq.gz## Overrepresented_sequences missing from 1B1Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 1B1Lardelli_R1.fq.gz## Overrepresented_sequences missing from 2B2Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 2B2Lardelli_R1.fq.gz## Overrepresented_sequences missing from 3D1Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 3D1Lardelli_R1.fq.gz## Overrepresented_sequences missing from 4D2Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 4D2Lardelli_R1.fq.gz## Overrepresented_sequences missing from 5F1Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 5F1Lardelli_R1.fq.gz## Overrepresented_sequences missing from 6F2Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 6F2Lardelli_R1.fq.gz## Overrepresented_sequences missing from 7G1Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 7G1Lardelli_R1.fq.gz## Overrepresented_sequences missing from 8G2Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 8G2Lardelli_R1.fq.gz## Overrepresented_sequences missing from 9H1Lardelli_2_R1.fq.gz## Overrepresented_sequences missing from 9H1Lardelli_R1.fq.gz## No overrepresented sequences were detected by FastQCR version 3.6.1 (2019-07-05)
Platform: x86_64-pc-linux-gnu (64-bit)
locale: LC_CTYPE=en_AU.UTF-8, LC_NUMERIC=C, LC_TIME=en_AU.UTF-8, LC_COLLATE=en_AU.UTF-8, LC_MONETARY=en_AU.UTF-8, LC_MESSAGES=en_AU.UTF-8, LC_PAPER=en_AU.UTF-8, LC_NAME=C, LC_ADDRESS=C, LC_TELEPHONE=C, LC_MEASUREMENT=en_AU.UTF-8 and LC_IDENTIFICATION=C
attached base packages: parallel, stats, graphics, grDevices, utils, datasets, methods and base
other attached packages: readr(v.1.3.1), stringr(v.1.4.0), magrittr(v.1.5), scales(v.1.0.0), pander(v.0.6.3), kableExtra(v.1.1.0), dplyr(v.0.8.3), ngsReports(v.1.1.1), tibble(v.2.1.3), ggplot2(v.3.2.0) and BiocGenerics(v.0.30.0)
loaded via a namespace (and not attached): bitops(v.1.0-6), matrixStats(v.0.54.0), lubridate(v.1.7.4), webshot(v.0.5.1), RColorBrewer(v.1.1-2), httr(v.1.4.0), GenomeInfoDb(v.1.20.0), tools(v.3.6.1), backports(v.1.1.4), R6(v.2.4.0), lazyeval(v.0.2.2), colorspace(v.1.4-1), withr(v.2.1.2), tidyselect(v.0.2.5), compiler(v.3.6.1), rvest(v.0.3.4), Biobase(v.2.44.0), Cairo(v.1.5-10), xml2(v.1.2.0), DelayedArray(v.0.10.0), plotly(v.4.9.0), ggdendro(v.0.1-20), labeling(v.0.3), digest(v.0.6.20), Rsamtools(v.2.0.0), rmarkdown(v.1.14), XVector(v.0.24.0), pkgconfig(v.2.0.2), htmltools(v.0.3.6), highr(v.0.8), htmlwidgets(v.1.3), rlang(v.0.4.0), rstudioapi(v.0.10), shiny(v.1.3.2), zoo(v.1.8-6), hwriter(v.1.3.2), jsonlite(v.1.6), crosstalk(v.1.0.0), BiocParallel(v.1.18.0), RCurl(v.1.95-4.12), GenomeInfoDbData(v.1.2.1), Matrix(v.1.2-17), Rcpp(v.1.0.1), munsell(v.0.5.0), S4Vectors(v.0.22.0), stringi(v.1.4.3), yaml(v.2.2.0), MASS(v.7.3-51.4), SummarizedExperiment(v.1.14.0), zlibbioc(v.1.30.0), plyr(v.1.8.4), grid(v.3.6.1), promises(v.1.0.1), crayon(v.1.3.4), lattice(v.0.20-38), Biostrings(v.2.52.0), hms(v.0.5.0), zeallot(v.0.1.0), knitr(v.1.23), pillar(v.1.4.2), GenomicRanges(v.1.36.0), reshape2(v.1.4.3), stats4(v.3.6.1), glue(v.1.3.1), evaluate(v.0.14), ShortRead(v.1.42.0), latticeExtra(v.0.6-28), data.table(v.1.12.2), vctrs(v.0.2.0), httpuv(v.1.5.1), gtable(v.0.3.0), purrr(v.0.3.2), tidyr(v.0.8.3), assertthat(v.0.2.1), xfun(v.0.8), mime(v.0.7), xtable(v.1.8-4), later(v.0.8.0), viridisLite(v.0.3.0), truncnorm(v.1.0-8), GenomicAlignments(v.1.20.1) and IRanges(v.2.18.1)